› 2xee

X-ray diffraction
2.1Å resolution

Structural Determinants for Improved Thermal Stability of Designed Ankyrin Repeat Proteins With a Redesigned C-capping Module.

Released:
DOI: 10.2210/pdb2xee/pdb
PDB entry 2xee coloured by chain, front view.
PDB entry 2xee coloured by chain, side view.
PDB entry 2xee coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module.
Kramer MA, Wetzel SK, Plückthun A, Mittl PR, Grütter MG
J Mol Biol 404 381-91 (2010)
PMID: 20851127

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NI3C DARPIN MUTANT5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 157 amino acids
Theoretical weight: 16.79 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 62.05Å b: 62.05Å c: 270.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.175 0.24
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
5 more

14 mentions without citation

Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display.
Könning et al. (2018)
13 more