› 2xeh

X-ray diffraction
1.81Å resolution

Structural Determinants for Improved Thermal Stability of Designed Ankyrin Repeat Proteins With a Redesigned C-capping Module.

Released:
DOI: 10.2210/pdb2xeh/pdb
PDB entry 2xeh coloured by chain, front view.
PDB entry 2xeh coloured by chain, side view.
PDB entry 2xeh coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module.
Kramer MA, Wetzel SK, Plückthun A, Mittl PR, Grütter MG
J Mol Biol 404 381-91 (2010)
PMID: 20851127

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NI3C MUT6 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 157 amino acids
Theoretical weight: 16.87 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P61
Unit cell:
a: 128.905Å b: 128.905Å c: 50.696Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.25
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
5 more

1 mention without citation

StaRProtein, a web server for prediction of the stability of repeat proteins.
Xu et al. (2015)