› 2xel

X-ray diffraction
2.5Å resolution

Molecular Mechanism of Pentachloropseudilin Mediated Inhibition of Myosin Motor Activity

Released:
DOI: 10.2210/pdb2xel/pdb
PDB entry 2xel coloured by chain, front view.
PDB entry 2xel coloured by chain, side view.
PDB entry 2xel coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Mechanism and specificity of pentachloropseudilin-mediated inhibition of myosin motor activity.
Chinthalapudi K, Taft MH, Martin R, Heissler SM, Preller M, Hartmann FK, Brandstaetter H, Kendrick-Jones J, Tsiavaliaris G, Gutzeit HO, Fedorov R, Buss F, Knölker HJ, Coluccio LM, Manstein DJ
J Biol Chem 286 29700-8 (2011)
PMID: 21680745

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin-2 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 776 amino acids
Theoretical weight: 88.51 KDa
Source organism: Dictyostelium discoideum
Expression system: Dictyostelium discoideum
UniProt:
  • Canonical: P08799 (Residues: 2-761; Coverage: 36%)
Gene names: DDB_G0286355, mhcA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand AD9 1 x AD9
Ligand MG 1 x MG
Ligand IA2 1 x IA2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 89.53Å b: 147.49Å c: 153.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.253
Expression system: Dictyostelium discoideum

Quick links

Citations

8 review citations

Nonmuscle myosin-2: mix and match.
Heissler et al. (2013)
7 more

8 mentions without citation

Kinetic Adaptations of Myosins for Their Diverse Cellular Functions.
Heissler et al. (2016)
7 more