› 2xen

X-ray diffraction
2.2Å resolution

Structural Determinants for Improved Thermal Stability of Designed Ankyrin Repeat Proteins With a Redesigned C-capping Module.

Released:
DOI: 10.2210/pdb2xen/pdb
PDB entry 2xen coloured by chain, front view.
PDB entry 2xen coloured by chain, side view.
PDB entry 2xen coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module.
Kramer MA, Wetzel SK, Plückthun A, Mittl PR, Grütter MG
J Mol Biol 404 381-91 (2010)
PMID: 20851127

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NI1C MUT4 Chain: A
Molecule details ›
Chain: A
Length: 91 amino acids
Theoretical weight: 9.73 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

3 bound ligands:
Ligand EDO 1 x EDO
Ligand MOH 1 x MOH
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 77.15Å b: 35.33Å c: 32.4Å
α: 90° β: 114.43° γ: 90°
R-values:
R R work R free
0.174 0.168 0.222
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
5 more

1 mention without citation

Thermostable designed ankyrin repeat proteins (DARPins) as building blocks for innovative drugs.
Schilling et al. (2022)