› 2xf1

X-ray diffraction
1.96Å resolution

Crystal structure of Plasmodium falciparum actin depolymerization factor 1

Released:
DOI: 10.2210/pdb2xf1/pdb
PDB entry 2xf1 coloured by chain, front view.
PDB entry 2xf1 coloured by chain, side view.
PDB entry 2xf1 coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Crystal structures explain functional differences in the two actin depolymerization factors of the malaria parasite.
Singh BK, Sattler JM, Chatterjee M, Huttu J, Schüler H, Kursula I
J Biol Chem 286 28256-64 (2011)
PMID: 21832095

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184669 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cofilin/actin-depolymerizing factor homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 14.18 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8I467 (Residues: 1-122; Coverage: 100%)
Gene name: PFE0165w
Sequence domains: Cofilin/tropomyosin-type actin-binding protein
Structure domains: Severin

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P3221
Unit cell:
a: 68.84Å b: 68.84Å c: 76.03Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Guardians of the actin monomer.
Xue et al. (2013)
4 more

4 mentions without citation

The apicomplexan glideosome and adhesins - Structures and function.
Boucher et al. (2015)
3 more