› 2xf8

X-ray diffraction
2.95Å resolution

Structure of the D-Erythrose-4-Phosphate Dehydrogenase from E. coli in complex with a NAD cofactor analog (3-Chloroacetyl adenine pyridine dinucleotide) and sulfate anion

Released:
DOI: 10.2210/pdb2xf8/pdb
PDB entry 2xf8 coloured by chain, front view.
PDB entry 2xf8 coloured by chain, side view.
PDB entry 2xf8 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Moniot S, Didierjean C, Boschi-Muller S, Branlant G, Corbier C

Function and Biology Details

Reaction catalysed: 
D-erythrose 4-phosphate + NAD(+) + H(2)O = 4-phosphoerythronate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141865 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-erythrose-4-phosphate dehydrogenase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 338 amino acids
Theoretical weight: 37.22 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A9B6 (Residues: 2-339; Coverage: 100%)
Gene names: JW2894, b2927, epd, gapB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand 3CD 8 x 3CD
1 bound ligand:
Ligand SO4 16 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P1
Unit cell:
a: 96.18Å b: 111.66Å c: 135.47Å
α: 91.3° β: 96.11° γ: 88.44°
R-values:
R R work R free
0.239 0.237 0.28
Expression system: Escherichia coli K-12

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