› 2xfg

X-ray diffraction
1.68Å resolution

Reassembly and co-crystallization of a family 9 processive endoglucanase from separately expressed GH9 and CBM3c modules

Released:
DOI: 10.2210/pdb2xfg/pdb
PDB entry 2xfg coloured by chain, front view.
PDB entry 2xfg coloured by chain, side view.
PDB entry 2xfg coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Reassembly and co-crystallization of a family 9 processive endoglucanase from its component parts: structural and functional significance of the intermodular linker.
Petkun S, Rozman Grinberg I, Lamed R, Jindou S, Burstein T, Yaniv O, Shoham Y, Shimon LJ, Bayer EA, Frolow F
PeerJ 3 e1126 (2015)
PMID: 26401442

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169783 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endoglucanase 1 Chain: A
Molecule details ›
Chain: A
Length: 466 amino acids
Theoretical weight: 52.82 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02934 (Residues: 54-516; Coverage: 55%)
Gene names: Cthe_0040, celI
Sequence domains: Glycosyl hydrolase family 9
Structure domains: Glycosyltransferase
Endoglucanase 1 Chain: B
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 19.58 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02934 (Residues: 517-683; Coverage: 20%)
Gene names: Cthe_0040, celI
Sequence domains: Cellulose binding domain
Structure domains: Endoglucanase-like

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 70.399Å b: 88.541Å c: 106.486Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.141 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Continually emerging mechanistic complexity of the multi-enzyme cellulosome complex.
Smith et al. (2017)
1 more

3 mentions without citation

Reassembly and co-crystallization of a family 9 processive endoglucanase from its component parts: structural and functional significance of the intermodular linker.
Petkun et al. (2015)
2 more