› 2xg9

X-ray diffraction
1.8Å resolution

Crystal structure of Barley Beta-Amylase complexed with 4-O-alpha-D- glucopyranosylmoranoline

Released:
DOI: 10.2210/pdb2xg9/pdb
PDB entry 2xg9 coloured by chain, front view.
PDB entry 2xg9 coloured by chain, side view.
PDB entry 2xg9 coloured by chain, top view.
Source organism: Hordeum vulgare
Primary publication:
Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley β-amylase.
Rejzek M, Stevenson CE, Southard AM, Stanley D, Denyer K, Smith AM, Naldrett MJ, Lawson DM, Field RA
Mol Biosyst 7 718-30 (2011)
PMID: 21085740

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147608 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-amylase Chain: A
Molecule details ›
Chain: A
Length: 535 amino acids
Theoretical weight: 59.67 KDa
Source organism: Hordeum vulgare
UniProt:
  • Canonical: P16098 (Residues: 1-535; Coverage: 100%)
Gene names: AMYB, BMY1
Sequence domains: Glycosyl hydrolase family 14
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand NOJ 1 x NOJ
Ligand GLC 1 x GLC
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 68.582Å b: 71.237Å c: 92.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.13 0.128 0.171

Quick links

Citations

2 review citations

A broader view: microbial enzymes and their relevance in industries, medicine, and beyond.
Gurung et al. (2013)
1 more

2 mentions without citation

Metagenomics of Atacama Lithobiontic Extremophile Life Unveils Highlights on Fungal Communities, Biogeochemical Cycles and Carbohydrate-Active Enzymes.
Gómez-Silva et al. (2019)
1 more