› 2xgl

X-ray diffraction
2.7Å resolution

The X-ray structure of the Escherichia coli colicin M immunity protein demonstrates the presence of a disulphide bridge, which is functionally essential

Released:
DOI: 10.2210/pdb2xgl/pdb
PDB entry 2xgl coloured by chain, front view.
PDB entry 2xgl coloured by chain, side view.
PDB entry 2xgl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
X-ray structure and site-directed mutagenesis analysis of the Escherichia coli colicin M immunity protein.
Gérard F, Brooks MA, Barreteau H, Touzé T, Graille M, Bouhss A, Blanot D, van Tilbeurgh H, Mengin-Lecreulx D
J Bacteriol 193 205-14 (2011)
PMID: 21037007

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148242 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-M immunity protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 12.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18002 (Residues: 48-141; Coverage: 67%)
Gene name: cmi
Sequence domains: YebF-like protein
Structure domains: YebF/Colicin-M immunity protein

Ligands and Environments

3 bound ligands:
Ligand CD 7 x CD
Ligand CL 11 x CL
Ligand NA 14 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 52Å b: 101.1Å c: 43.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.268
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Ribosomally encoded antibacterial proteins and peptides from Pseudomonas.
Ghequire et al. (2014)
3 more

1 mention without citation

X-ray structure and site-directed mutagenesis analysis of the Escherichia coli colicin M immunity protein.
Gérard et al. (2011)