› 2xgp

X-ray diffraction
2.7Å resolution

Yeast DNA polymerase eta in complex with C8-2-acetylaminofluorene containing DNA

Released:
DOI: 10.2210/pdb2xgp/pdb
PDB entry 2xgp coloured by chain, front view.
PDB entry 2xgp coloured by chain, side view.
PDB entry 2xgp coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Mechanism of replication blocking and bypass of Y-family polymerase {eta} by bulky acetylaminofluorene DNA adducts.
Schorr S, Schneider S, Lammens K, Hopfner KP, Carell T
Proc Natl Acad Sci U S A 107 20720-5 (2010)
PMID: 21076032

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115928 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase eta Chains: A, B
Molecule details ›
Chains: A, B
Length: 536 amino acids
Theoretical weight: 60.71 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04049 (Residues: 1-513; Coverage: 81%)
Gene names: DBH1, RAD30, YDR419W
Sequence domains: impB/mucB/samB family
Structure domains:
5'-D(*GP*TP*GP*GP*AP*TP*GP*AP*G)-3' Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 nucleotides
Theoretical weight: 2.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(*CP*8FG*CP*TP*CP*AP*TP*CP*CP*AP*C)-3' Chains: T, U
Molecule details ›
Chains: T, U
Length: 11 nucleotides
Theoretical weight: 3.48 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
1 modified residue:
Ligand 8FG 2 x 8FG

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 103.44Å b: 103.44Å c: 292.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.272
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Genotoxic anti-cancer agents and their relationship to DNA damage, mitosis, and checkpoint adaptation in proliferating cancer cells.
Swift et al. (2014)
3 more

2 mentions without citation

Mechanism of replication blocking and bypass of Y-family polymerase {eta} by bulky acetylaminofluorene DNA adducts.
Schorr et al. (2010)
1 more