2xh0

X-ray diffraction
1.7Å resolution

Engineering the enolase active site pocket: Crystal structure of the S39N Q167K D321R mutant of yeast enolase 1

Released:
Source organism: Saccharomyces cerevisiae

Function and Biology Details

Reaction catalysed:
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 443 amino acids
Theoretical weight: 47.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00924 (Residues: 2-437; Coverage: 100%)
Gene names: ENO1, ENOA, G9160, HSP48, YGR254W
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 55.329Å b: 60.797Å c: 120.667Å
α: 89.89° β: 89.9° γ: 65.84°
R-values:
R R work R free
0.199 0.197 0.246
Expression system: Escherichia coli BL21