› 2xhh

X-ray diffraction
1.6Å resolution

Circular permutation provides an evolutionary link between two families of calcium-dependent carbohydrate binding modules

Released:
DOI: 10.2210/pdb2xhh/pdb
PDB entry 2xhh coloured by chain, front view.
PDB entry 2xhh coloured by chain, side view.
PDB entry 2xhh coloured by chain, top view.
Source organism: Cellvibrio japonicus
Primary publication:
Circular permutation provides an evolutionary link between two families of calcium-dependent carbohydrate binding modules.
Montanier C, Flint JE, Bolam DN, Xie H, Liu Z, Rogowski A, Weiner DP, Ratnaparkhe S, Nurizzo D, Roberts SM, Turkenburg JP, Davies GJ, Gilbert HJ
J Biol Chem 285 31742-54 (2010)
PMID: 20659893

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CARBOHYDRATE BINDING MODULE Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 12.88 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli
Structure domains: Lipoxygenase-1

Ligands and Environments

2 bound ligands:
Ligand LMR 1 x LMR
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 45.366Å b: 45.366Å c: 102.259Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 0.116 0.18
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules.
Gilbert et al. (2013)
3 more

3 mentions without citation

Circular permutation provides an evolutionary link between two families of calcium-dependent carbohydrate binding modules.
Montanier et al. (2010)
2 more