› 2xi8

X-ray diffraction
1.21Å resolution

High resolution structure of native CylR2

Released:
DOI: 10.2210/pdb2xi8/pdb
PDB entry 2xi8 coloured by chain, front view.
PDB entry 2xi8 coloured by chain, side view.
PDB entry 2xi8 coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Integrated analysis of the conformation of a protein-linked spin label by crystallography, EPR and NMR spectroscopy.
Gruene T, Cho MK, Karyagina I, Kim HY, Grosse C, Giller K, Zweckstetter M, Becker S
J Biomol NMR 49 111-9 (2011)
PMID: 21271275

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.72 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8VL32 (Residues: 1-66; Coverage: 100%)
Gene names: EU507_14965, cylR2
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41
Unit cell:
a: 63.412Å b: 63.412Å c: 41.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 not available 0.167
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The magic of bicelles lights up membrane protein structure.
Dürr et al. (2012)
4 more

2 mentions without citation

Integrated analysis of the conformation of a protein-linked spin label by crystallography, EPR and NMR spectroscopy.
Gruene et al. (2011)
1 more