› 2xiw

X-ray diffraction
1.5Å resolution

Crystal structure of the Sac7d-derived IgG1-binder C3-C24S

Released:
DOI: 10.2210/pdb2xiw/pdb
PDB entry 2xiw coloured by chain, front view.
PDB entry 2xiw coloured by chain, side view.
PDB entry 2xiw coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius
Primary publication:
Tolerance of the archaeal Sac7d scaffold protein to alternative library designs: characterization of anti-immunoglobulin G Affitins.
Béhar G, Bellinzoni M, Maillasson M, Paillard-Laurance L, Alzari PM, He X, Mouratou B, Pecorari F
Protein Eng Des Sel 26 267-75 (2013)
PMID: 23315487

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein 7d Chains: A, B
Molecule details ›
Chains: A, B
Length: 79 amino acids
Theoretical weight: 9.17 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: P13123 (Residues: 2-66; Coverage: 99%)
Gene name: Saci_0064
Sequence domains: 7kD DNA-binding domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3121
Unit cell:
a: 73.175Å b: 73.175Å c: 57.445Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.191
Expression system: Escherichia coli DH5[alpha]

Quick links

Citations

6 review citations

Designer protein delivery: From natural to engineered affinity-controlled release systems.
Pakulska et al. (2016)
5 more

1 mention without citation

Miniproteins as a Powerful Modality in Drug Development.
Crook et al. (2020)