› 2xkp

X-ray diffraction
3.05Å resolution

NtcA from Synechococcus elongatus: active and inactive

Released:
DOI: 10.2210/pdb2xkp/pdb
PDB entry 2xkp coloured by chain, front view.
PDB entry 2xkp coloured by chain, side view.
PDB entry 2xkp coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
Structural basis for the regulation of NtcA-dependent transcription by proteins PipX and PII.
Llácer JL, Espinosa J, Castells MA, Contreras A, Forchhammer K, Rubio V
Proc Natl Acad Sci U S A 107 15397-402 (2010)
PMID: 20716687

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Global nitrogen regulator Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 222 amino acids
Theoretical weight: 24.86 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli
UniProt:
  • Canonical: P29283 (Residues: 1-222; Coverage: 100%)
Gene names: Synpcc7942_0127, ntcA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AKG 6 x AKG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P61
Unit cell:
a: 110.391Å b: 110.391Å c: 219.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.247
Expression system: Escherichia coli

Quick links

Citations

17 review citations

The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite.
Huergo et al. (2015)
16 more

1 mention without citation

Structural basis for the regulation of NtcA-dependent transcription by proteins PipX and PII.
Llácer et al. (2010)