› 2xkv

Electron Microscopy
13.5Å resolution

Atomic Model of the SRP-FtsY Early Conformation

Released:
DOI: 10.2210/pdb2xkv/pdb
PDB entry 2xkv coloured by chain, front view.
PDB entry 2xkv coloured by chain, side view.
PDB entry 2xkv coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor.
Estrozi LF, Boehringer D, Shan SO, Ban N, Schaffitzel C
Nat Struct Mol Biol 18 88-90 (2011)
PMID: 21151118
Related structures: EMD-1762

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126577 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 32.3 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: O07347 (Residues: 1-294; Coverage: 68%)
Gene name: ffh
Sequence domains:
Signal recognition particle protein Chain: C
Molecule details ›
Chain: C
Length: 69 amino acids
Theoretical weight: 8.16 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AGD7 (Residues: 368-430; Coverage: 14%)
Gene names: JW5414, b2610, ffh
Sequence domains: Signal peptide binding domain
Signal recognition particle receptor FtsY Chain: D
Molecule details ›
Chain: D
Length: 303 amino acids
Theoretical weight: 32.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P83749 (Residues: 2-304; Coverage: 100%)
Gene name: ftsY
Sequence domains:
4.5S RNA Chain: B
Molecule details ›
Chain: B
Length: 114 nucleotides
Theoretical weight: 36.86 KDa
Sequence domains: Bacterial small signal recognition particle RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 13.5Å
Relevant EMDB volumes: EMD-1762
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The noncoding RNA revolution-trashing old rules to forge new ones.
Cech et al. (2014)
11 more

7 mentions without citation

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
6 more