› 2xlc

X-ray diffraction
2.7Å resolution

Function and Biology Details

Reaction catalysed: 
An acetic ester + H(2)O = an alcohol + acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191766 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl xylan esterase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 320 amino acids
Theoretical weight: 36.34 KDa
Source organism: Bacillus pumilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K5F2 (Residues: 1-320; Coverage: 100%)
Gene name: axe
Sequence domains: Acetyl xylan esterase (AXE1)
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand DEP 6 x DEP
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P21
Unit cell:
a: 82.049Å b: 116.052Å c: 100.248Å
α: 90° β: 105.6° γ: 90°
R-values:
R R work R free
0.217 0.216 0.236
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Properties and biotechnological applications of microbial deacetylase.
Zou et al. (2023)
11 other articles

1 mention without citation

Understanding the structural and functional properties of carbohydrate esterases with a special focus on hemicellulose deacetylating acetyl xylan esterases.
Sista Kameshwar et al. (2018)