2xlk

X-ray diffraction
1.8Å resolution

Crystal structure of the Csy4-crRNA complex, orthorhombic form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-169801 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endonuclease Cas6/Csy4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.87 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
Structure domains: CRISPR-associated endoribonuclease Cas6/Csy4
5'-R(*CP*UP*GP*CP*CP*GP*UP*AP*UP*AP*GP*GP*CP*A*DG*C)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 5.1 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 40.06Å b: 78.91Å c: 145.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.216
Expression systems:
  • Escherichia coli BL21
  • Not provided