› 2xma

X-ray diffraction
2.3Å resolution

DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE RIGHT END DNA COMPLEX

Released:
DOI: 10.2210/pdb2xma/pdb
PDB entry 2xma coloured by chain, front view.
PDB entry 2xma coloured by chain, side view.
PDB entry 2xma coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans.
Hickman AB, James JA, Barabas O, Pasternak C, Ton-Hoang B, Chandler M, Sommer S, Dyda F
EMBO J 29 3840-52 (2010)
PMID: 20890269

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115934 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
ISDra2 transposase TnpA Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 143 amino acids
Theoretical weight: 16.42 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7DF83 (Residues: 1-140; Coverage: 100%)
Gene names: DR_0667, DR_0979, DR_1382, DR_1592, DR_1652, DR_1932, DR_2323, orf1, tnpA, tnpB
Sequence domains: Transposase IS200 like
Structure domains: Transposase IS200-like
DRA2 TRANSPOSASE RIGHT END RECOGNITION SITE Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 34 nucleotides
Theoretical weight: 10.54 KDa
Source organism: Deinococcus radiodurans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 76.827Å b: 89.288Å c: 90.104Å
α: 90° β: 111.23° γ: 90°
R-values:
R R work R free
0.215 0.215 0.238
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

Bacterial insertion sequences: their genomic impact and diversity.
Siguier et al. (2014)
7 more

1 mention without citation

DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans.
Hickman et al. (2010)