› 2xmw

X-ray diffraction
1.8Å resolution

PacS, N-terminal domain, from Synechocystis PCC6803

Released:
DOI: 10.2210/pdb2xmw/pdb
PDB entry 2xmw coloured by chain, front view.
PDB entry 2xmw coloured by chain, side view.
PDB entry 2xmw coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Visualizing the metal-binding versatility of copper trafficking sites .
Badarau A, Firbank SJ, McCarthy AA, Banfield MJ, Dennison C
Biochemistry 49 7798-810 (2010)
PMID: 20726513

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Cu(+)(Side 1) = ADP + phosphate + Cu(+)(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable copper-transporting ATPase PacS Chain: A
Molecule details ›
Chain: A
Length: 71 amino acids
Theoretical weight: 7.65 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P73241 (Residues: 1-71; Coverage: 10%)
Gene names: pacS, sll1920
Sequence domains: Heavy-metal-associated domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand CU1 1 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P321
Unit cell:
a: 45.58Å b: 45.58Å c: 51.831Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.243 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Single-molecule dynamics and mechanisms of metalloregulators and metallochaperones.
Chen et al. (2013)
1 more

1 mention without citation

Investigating the role of zinc and copper binding motifs of trafficking sites in the cyanobacterium Synechocystis PCC 6803.
Badarau et al. (2013)