› 2xmx

X-ray diffraction
1.67Å resolution

High resolution structure of Colicin M

Released:
DOI: 10.2210/pdb2xmx/pdb
PDB entry 2xmx coloured by chain, front view.
PDB entry 2xmx coloured by chain, side view.
PDB entry 2xmx coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Activation of colicin M by the FkpA prolyl cis-trans isomerase/chaperone.
Helbig S, Patzer SI, Schiene-Fischer C, Zeth K, Braun V
J Biol Chem 286 6280-90 (2011)
PMID: 21149455

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-M Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 29.51 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05820 (Residues: 1-271; Coverage: 100%)
Gene name: cma
Sequence domains: Colicin M
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CO3 3 x CO3
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 52.757Å b: 115.007Å c: 227.714Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.157 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The Bam machine: a molecular cooper.
Ricci et al. (2012)
7 more

7 mentions without citation

The Biology of Colicin M and Its Orthologs.
Chérier et al. (2021)
6 more