› 2xn2

X-ray diffraction
1.58Å resolution

Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with galactose

Released:
DOI: 10.2210/pdb2xn2/pdb
PDB entry 2xn2 coloured by chain, front view.
PDB entry 2xn2 coloured by chain, side view.
PDB entry 2xn2 coloured by chain, top view.
Source organism: Lactobacillus acidophilus NCFM
Primary publication:
Crystal structure of α-galactosidase from Lactobacillus acidophilus NCFM: insight into tetramer formation and substrate binding.
Fredslund F, Hachem MA, Larsen RJ, Sørensen PG, Coutinho PM, Lo Leggio L, Svensson B
J Mol Biol 412 466-80 (2011)
PMID: 21827767

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-galactosidase Mel36A Chain: A
Molecule details ›
Chain: A
Length: 732 amino acids
Theoretical weight: 84.62 KDa
Source organism: Lactobacillus acidophilus NCFM
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G1UB44 (Residues: 1-732; Coverage: 100%)
Gene names: LBA1438, aga, melA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLA 1 x GLA
Ligand IMD 1 x IMD
Ligand GOL 25 x GOL
1 modified residue:
Ligand SME 2 x SME

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: I222
Unit cell:
a: 91.61Å b: 126.7Å c: 148.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 0.131 0.155
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Biotechnological potential of microbial α-galactosidases.
Katrolia et al. (2014)
3 more

6 mentions without citation

Game on, science - how video game technology may help biologists tackle visualization challenges.
Lv et al. (2013)
5 more