› 2xnf

Solution NMR

The Mediator Med25 activator interaction domain: Structure and cooperative binding of VP16 subdomains

Released:
DOI: 10.2210/pdb2xnf/pdb
PDB entry 2xnf coloured by chain, ensemble of 10 models, front view.
PDB entry 2xnf coloured by chain, ensemble of 10 models, side view.
PDB entry 2xnf coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure and VP16 binding of the Mediator Med25 activator interaction domain.
Vojnic E, Mourão A, Seizl M, Simon B, Wenzeck L, Larivière L, Baumli S, Baumgart K, Meisterernst M, Sattler M, Cramer P
Nat Struct Mol Biol 18 404-9 (2011)
PMID: 21378965

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mediator of RNA polymerase II transcription subunit 25 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q71SY5 (Residues: 394-543; Coverage: 20%)
Gene names: ACID1, ARC92, MED25, PTOV2, TCBAP0758
Sequence domains: Mediator complex subunit 25 PTOV activation and synapsin 2
Structure domains: Mediator complex subunit 25, ACID domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: CYANA
Expression system: Escherichia coli

Quick links

Citations

19 review citations

The Mediator complex: a central integrator of transcription.
Allen et al. (2015)
18 more

10 mentions without citation

SPOC domain proteins in health and disease.
Appel et al. (2023)
9 more