› 2xnk

X-ray diffraction
2.6Å resolution

Structure and function of the Rad9-binding region of the DNA damage checkpoint adaptor TopBP1

Released:
DOI: 10.2210/pdb2xnk/pdb
PDB entry 2xnk coloured by chain, front view.
PDB entry 2xnk coloured by chain, side view.
PDB entry 2xnk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and function of the Rad9-binding region of the DNA-damage checkpoint adaptor TopBP1.
Rappas M, Oliver AW, Pearl LH
Nucleic Acids Res 39 313-24 (2011)
PMID: 20724438

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2-binding protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 292 amino acids
Theoretical weight: 34.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92547 (Residues: 1-290; Coverage: 19%)
Gene names: KIAA0259, TOPBP1
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain

Ligands and Environments

1 bound ligand:
Ligand GOL 11 x GOL
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 39.49Å b: 290.28Å c: 60.58Å
α: 90° β: 89.85° γ: 90°
R-values:
R R work R free
0.208 0.205 0.266
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Phospho-Ser/Thr-binding domains: navigating the cell cycle and DNA damage response.
Reinhardt et al. (2013)
10 more

1 mention without citation

BRCT domains: easy as one, two, three.
Leung et al. (2011)