› 2xon

X-ray diffraction
1.4Å resolution

Structure of TmCBM61 in complex with beta-1,4-galactotriose at 1.4 A resolution

Released:
DOI: 10.2210/pdb2xon/pdb
PDB entry 2xon coloured by chain, front view.
PDB entry 2xon coloured by chain, side view.
PDB entry 2xon coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Recognition of the helical structure of beta-1,4-galactan by a new family of carbohydrate-binding modules.
Cid M, Pedersen HL, Kaneko S, Coutinho PM, Henrissat B, Willats WG, Boraston AB
J Biol Chem 285 35999-6009 (2010)
PMID: 20826814

Function and Biology Details

Reaction catalysed: 
The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194739 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Arabinogalactan endo-beta-1,4-galactanase Chains: A, L
Molecule details ›
Chains: A, L
Length: 152 amino acids
Theoretical weight: 17.22 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9X0S8 (Residues: 461-606; Coverage: 24%)
Gene name: TM_1201
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand SO4 2 x SO4
Ligand EDO 25 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 48.9Å b: 61.85Å c: 55.53Å
α: 90° β: 105.14° γ: 90°
R-values:
R R work R free
0.119 0.116 0.167
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules.
Gilbert et al. (2013)
7 more

4 mentions without citation

Recognition of the helical structure of beta-1,4-galactan by a new family of carbohydrate-binding modules.
Cid et al. (2010)
3 more