› 2xoy

X-ray diffraction
2.6Å resolution

C-terminal cysteine-rich domain of human CHFR bound to P(1),P(2)- Diadenosine-5'-pyrophosphate

Released:
DOI: 10.2210/pdb2xoy/pdb
PDB entry 2xoy coloured by chain, front view.
PDB entry 2xoy coloured by chain, side view.
PDB entry 2xoy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of poly(ADP-ribose) recognition by the multizinc binding domain of checkpoint with forkhead-associated and RING Domains (CHFR).
Oberoi J, Richards MW, Crumpler S, Brown N, Blagg J, Bayliss R
J Biol Chem 285 39348-58 (2010)
PMID: 20880844

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase CHFR Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 29.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96EP1 (Residues: 407-664; Coverage: 39%)
Gene names: CHFR, RNF196
Sequence domains: Cysteine rich domain with multizinc binding regions
Structure domains: Herpes Virus-1

Ligands and Environments

3 bound ligands:
Ligand ZN 10 x ZN
Ligand AMP 1 x AMP
Ligand ADE 1 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 163.05Å b: 52.12Å c: 83.61Å
α: 90° β: 105.34° γ: 90°
R-values:
R R work R free
0.199 0.195 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

New insights into the molecular and cellular functions of poly(ADP-ribose) and PARPs.
Gibson et al. (2012)
22 more

3 mentions without citation

PARPs and ADP-ribosylation: recent advances linking molecular functions to biological outcomes.
Gupte et al. (2017)
2 more