› 2xqh

X-ray diffraction
1.99Å resolution

Crystal structure of an immunoglobulin-binding fragment of the trimeric autotransporter adhesin EibD

Released:
DOI: 10.2210/pdb2xqh/pdb
PDB entry 2xqh coloured by chain, front view.
PDB entry 2xqh coloured by chain, side view.
PDB entry 2xqh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of E. coli IgG-binding protein D suggests a general model for bending and binding in trimeric autotransporter adhesins.
Leo JC, Lyskowski A, Hattula K, Hartmann MD, Schwarz H, Butcher SJ, Linke D, Lupas AN, Goldman A
Structure 19 1021-30 (2011)
PMID: 21742268

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin-binding protein EibD Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 29.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9MCI8 (Residues: 160-418; Coverage: 53%)
Gene name: eibD
Sequence domains: YadA head domain repeat (2 copies)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: R3
Unit cell:
a: 48.95Å b: 48.95Å c: 409.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.158 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Type V secretion: mechanism(s) of autotransport through the bacterial outer membrane.
Leo et al. (2012)
10 more

6 mentions without citation

Looks can be deceiving: recent insights into the mechanism of protein secretion by the autotransporter pathway.
Bernstein HD. (2015)
5 more