› 2xs3

X-ray diffraction
2.4Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111968 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Karilysin short form Kly18 Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 18.7 KDa
Source organism: Tannerella forsythia
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: D0EM77 (Residues: 35-200; Coverage: 37%)
Gene names: BFO_2683, kly
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)
PEPTIDE ALA-PHE-THR-SER Chains: C, D
Molecule details ›
Chains: C, D
Length: 4 amino acids
Theoretical weight: 424 Da
Source organism: synthetic construct

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 121.37Å b: 53.11Å c: 86.3Å
α: 90° β: 134.55° γ: 90°
R-values:
R R work R free
0.192 0.189 0.259
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

The Type IX Secretion System (T9SS): Highlights and Recent Insights into Its Structure and Function.
Lasica et al. (2017)
1 more

3 mentions without citation

The structure of the catalytic domain of Tannerella forsythia karilysin reveals it is a bacterial xenologue of animal matrix metalloproteinases.
Cerdà-Costa et al. (2011)
2 more