› 2xsf

X-ray diffraction
1.7Å resolution

Crystal structure of the RRM domain of mouse Deleted in azoospermia- like

Released:
DOI: 10.2210/pdb2xsf/pdb
PDB entry 2xsf coloured by chain, front view.
PDB entry 2xsf coloured by chain, side view.
PDB entry 2xsf coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Kinked β-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL.
Jenkins HT, Malkova B, Edwards TA
Proc Natl Acad Sci U S A 108 18266-71 (2011)
PMID: 22021443

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179126 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deleted in azoospermia-like Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 9.9 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q64368 (Residues: 35-118; Coverage: 28%)
Gene names: Dazl, Dazl1, Dazla
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P3221
Unit cell:
a: 53.9Å b: 53.9Å c: 65.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.189 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

RRM-RNA recognition: NMR or crystallography…and new findings.
Daubner et al. (2013)
9 more

1 mention without citation

Kinked β-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL.
Jenkins et al. (2011)