› 2xt0

X-ray diffraction
1.9Å resolution

Dehalogenase DPpA from Plesiocystis pacifica SIR-I

Released:
DOI: 10.2210/pdb2xt0/pdb
PDB entry 2xt0 coloured by chain, front view.
PDB entry 2xt0 coloured by chain, side view.
PDB entry 2xt0 coloured by chain, top view.
Source organism: Plesiocystis pacifica
Primary publication:
Cloning, functional expression, biochemical characterization, and structural analysis of a haloalkane dehalogenase from Plesiocystis pacifica SIR-1.
Hesseler M, Bogdanović X, Hidalgo A, Berenguer J, Palm GJ, Hinrichs W, Bornscheuer UT
Appl Microbiol Biotechnol 91 1049-60 (2011)
PMID: 21603934

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-107840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 32.62 KDa
Source organism: Plesiocystis pacifica
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A6G7B1 (Residues: 1-297; Coverage: 100%)
Gene name: PPSIR1_08671
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21212
Unit cell:
a: 47.262Å b: 108.501Å c: 67.312Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.248
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Haloalkane dehalogenases: biotechnological applications.
Koudelakova et al. (2013)
5 more

4 mentions without citation

Biochemical characterization of two haloalkane dehalogenases: DccA from Caulobacter crescentus and DsaA from Saccharomonospora azurea.
Carlucci et al. (2016)
3 more