› 2xt1

X-ray diffraction
1.32Å resolution

Crystal structure of the HIV-1 capsid protein C-terminal domain (146- 231) in complex with a camelid VHH.

Released:
DOI: 10.2210/pdb2xt1/pdb
PDB entry 2xt1 coloured by chain, front view.
PDB entry 2xt1 coloured by chain, side view.
PDB entry 2xt1 coloured by chain, top view.
Source organisms:
Entry authors: Igonet S, Vaney MC, Bartonova V, Helma J, Rothbauer U, Leonhardt H, Stura E, Krausslich H-G, Rey FA

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212433 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Retroviral nucleocapsid Gag protein p24 C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.53 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q71B32 (Residues: 146-231; Coverage: 37%)
Gene name: gag
Sequence domains: Gag protein p24 C-terminal domain
Structure domains: Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A
CAMELID VHH 9 Chain: B
Molecule details ›
Chain: B
Length: 121 amino acids
Theoretical weight: 13.21 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 60.06Å b: 88.17Å c: 35.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.184
Expression system: Escherichia coli BL21(DE3)

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