› 2xut

X-ray diffraction
3.62Å resolution

Crystal structure of a proton dependent oligopeptide (POT) family transporter.

Released:
DOI: 10.2210/pdb2xut/pdb
PDB entry 2xut coloured by chain, front view.
PDB entry 2xut coloured by chain, side view.
PDB entry 2xut coloured by chain, top view.
Source organism: Shewanella oneidensis MR-1
Primary publication:
Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2.
Newstead S, Drew D, Cameron AD, Postis VL, Xia X, Fowler PW, Ingram JC, Carpenter EP, Sansom MS, McPherson MJ, Baldwin SA, Iwata S
EMBO J 30 417-26 (2011)
PMID: 21131908

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione uptake transporter Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 524 amino acids
Theoretical weight: 57.68 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8EKT7 (Residues: 1-516; Coverage: 100%)
Gene name: SO_0002
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P32
Unit cell:
a: 159.4Å b: 159.4Å c: 152.94Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.278 0.276 0.296
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

31 review citations

The ABCs of membrane transporters in health and disease (SLC series): introduction.
Hediger et al. (2013)
30 more

38 mentions without citation

Influences of membrane mimetic environments on membrane protein structures.
Zhou et al. (2013)
37 more