› 2xv0

X-ray diffraction
1.6Å resolution

Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAHAAM), chemically reduced, pH4.8

Released:
DOI: 10.2210/pdb2xv0/pdb
PDB entry 2xv0 coloured by chain, front view.
PDB entry 2xv0 coloured by chain, side view.
PDB entry 2xv0 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Metal-binding loop length is a determinant of the pKa of a histidine ligand at a type 1 copper site.
Li C, Sato K, Monari S, Salard I, Sola M, Banfield MJ, Dennison C
Inorg Chem 50 482-8 (2011)
PMID: 21141901

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.57 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 98%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU1 1 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 39.987Å b: 46.149Å c: 57.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.199
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Blue Copper Proteins: A rigid machine for efficient electron transfer, a flexible device for metal uptake.
Pérez-Henarejos et al. (2015)
1 other article