› 2xvm

X-ray diffraction
1.48Å resolution

Crystal structure of the tellurite detoxification protein TehB from E. coli in complex with SAH

Released:
DOI: 10.2210/pdb2xvm/pdb
PDB entry 2xvm coloured by chain, front view.
PDB entry 2xvm coloured by chain, side view.
PDB entry 2xvm coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and mechanism of the chalcogen-detoxifying protein TehB from Escherichia coli.
Choudhury HG, Cameron AD, Iwata S, Beis K
Biochem J 435 85-91 (2011)
PMID: 21244361

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + tellurite = S-adenosyl-L-homocysteine + methanetelluronate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150458 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tellurite methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 199 amino acids
Theoretical weight: 22.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25397 (Residues: 1-197; Coverage: 100%)
Gene names: JW1426, b1430, tehB
Sequence domains: Tellurite resistance protein TehB
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 130.362Å b: 55.644Å c: 59.42Å
α: 90° β: 97.72° γ: 90°
R-values:
R R work R free
0.173 0.17 0.23
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Biomineralization of selenium by the selenate-respiring bacterium Thauera selenatis.
Butler et al. (2012)
1 more