2xwl

X-ray diffraction
1.49Å resolution

Crystal structure of IspD from Mycobacterium smegmatis in complex with CTP and Mg

Released:
Source organism: Mycolicibacterium smegmatis
Primary publication:
Structural and functional studies of mycobacterial IspD enzymes.
Acta Crystallogr D Biol Crystallogr 67 403-14 (2011)
PMID: 21543842

Function and Biology Details

Reaction catalysed:
CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-106464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 22.67 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0R560 (Residues: 1-219; Coverage: 99%)
Gene names: MSMEG_6076, ispD
Sequence domains: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 45.33Å b: 72.98Å c: 117.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.21
Expression system: Escherichia coli BL21