› 2xwo

X-ray diffraction
1.54Å resolution

SiaP R147E mutant in complex with sialylamide

Released:
DOI: 10.2210/pdb2xwo/pdb
PDB entry 2xwo coloured by chain, front view.
PDB entry 2xwo coloured by chain, side view.
PDB entry 2xwo coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Tripartite ATP-independent Periplasmic (TRAP) Transporters Use an Arginine-mediated Selectivity Filter for High Affinity Substrate Binding.
Fischer M, Hopkins AP, Severi E, Hawkhead J, Bawdon D, Watts AG, Hubbard RE, Thomas GH
J Biol Chem 290 27113-27123 (2015)
PMID: 26342690

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155185 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialic acid-binding periplasmic protein SiaP Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 35 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P44542 (Residues: 24-329; Coverage: 100%)
Gene names: HI_0146, siaP
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

1 bound ligand:
Ligand SLM 1 x SLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 47.86Å b: 74.76Å c: 87.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.13 0.179
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Tripartite ATP-Independent Periplasmic (TRAP) Transporters and Tripartite Tricarboxylate Transporters (TTT): From Uptake to Pathogenicity.
Rosa et al. (2018)
2 more

1 mention without citation

Tripartite ATP-independent Periplasmic (TRAP) Transporters Use an Arginine-mediated Selectivity Filter for High Affinity Substrate Binding.
Fischer et al. (2015)