› 2y08

X-ray diffraction
1.7Å resolution

Structure of the substrate-free FAD-dependent tirandamycin oxidase TamL

Released:
DOI: 10.2210/pdb2y08/pdb
PDB entry 2y08 coloured by chain, front view.
PDB entry 2y08 coloured by chain, side view.
PDB entry 2y08 coloured by chain, top view.
Source organism: Streptomyces sp. 307-9
Primary publication:
Tirandamycin biosynthesis is mediated by co-dependent oxidative enzymes.
Carlson JC, Li S, Gunatilleke SS, Anzai Y, Burr DA, Podust LM, Sherman DH
Nat Chem 3 628-33 (2011)
PMID: 21778983

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding PCMH-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 530 amino acids
Theoretical weight: 58.19 KDa
Source organism: Streptomyces sp. 307-9
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D3Y1I2 (Residues: 1-500; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FAD 2 x FAD
Ligand MG 2 x MG
Ligand GOL 8 x GOL
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 64.378Å b: 129.558Å c: 134.585Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.141 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.
Rudolf et al. (2017)
6 more

2 mentions without citation

Tirandamycin biosynthesis is mediated by co-dependent oxidative enzymes.
Carlson et al. (2011)
1 more