› 2y0r

X-ray diffraction
2.85Å resolution

Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2

Released:
DOI: 10.2210/pdb2y0r/pdb
PDB entry 2y0r coloured by chain, front view.
PDB entry 2y0r coloured by chain, side view.
PDB entry 2y0r coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Structural basis for the allosteric interference of myosin function by reactive thiol region mutations G680A and G680V.
Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ
J Biol Chem 286 35051-60 (2011)
PMID: 21841195

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin-2 heavy chain Chain: X
Molecule details ›
Chain: X
Length: 758 amino acids
Theoretical weight: 86.38 KDa
Source organism: Dictyostelium discoideum
Expression system: Dictyostelium discoideum
UniProt:
  • Canonical: P08799 (Residues: 2-759; Coverage: 36%)
Gene names: DDB_G0286355, mhcA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Unit cell:
a: 54.6Å b: 106Å c: 178.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.253 0.301
Expression system: Dictyostelium discoideum

Quick links

Citations

11 citation in other articles

Mammalian myosin-18A, a highly divergent myosin.
Guzik-Lendrum et al. (2013)
10 more

2 mentions without citation

Structural basis for the allosteric interference of myosin function by reactive thiol region mutations G680A and G680V.
Preller et al. (2011)
1 more