› 2y30

X-ray diffraction
2.3Å resolution

Simocyclinone D8 bound form of TetR-like repressor SimR

Released:
DOI: 10.2210/pdb2y30/pdb
PDB entry 2y30 coloured by chain, front view.
PDB entry 2y30 coloured by chain, side view.
PDB entry 2y30 coloured by chain, top view.
Source organism: Streptomyces antibioticus
Primary publication:
Structures of the TetR-like simocyclinone efflux pump repressor, SimR, and the mechanism of ligand-mediated derepression.
Le TB, Stevenson CE, Fiedler HP, Maxwell A, Lawson DM, Buttner MJ
J Mol Biol 408 40-56 (2011)
PMID: 21354180

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189591 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 30.24 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AMH9 (Residues: 3-261; Coverage: 99%)
Gene names: sim16, simReg2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SM8 2 x SM8
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 147.37Å b: 83.91Å c: 45.47Å
α: 90° β: 90.94° γ: 90°
R-values:
R R work R free
0.203 0.2 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The TetR family of regulators.
Cuthbertson et al. (2013)
3 more

4 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
3 more