› 2y3g

X-ray diffraction
1.91Å resolution

Se-Met form of Cupriavidus metallidurans CH34 CnrXs

Released:
DOI: 10.2210/pdb2y3g/pdb
PDB entry 2y3g coloured by chain, front view.
PDB entry 2y3g coloured by chain, side view.
PDB entry 2y3g coloured by chain, top view.
Source organism: Cupriavidus metallidurans CH34
Primary publication:
Structural basis for metal sensing by CnrX.
Trepreau J, Girard E, Maillard AP, de Rosny E, Petit-Haertlein I, Kahn R, Covès J
J Mol Biol 408 766-79 (2011)
PMID: 21414325

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nickel and cobalt resistance protein CnrR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 118 amino acids
Theoretical weight: 13.36 KDa
Source organism: Cupriavidus metallidurans CH34
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37975 (Residues: 31-148; Coverage: 97%)
Gene names: RMe0087, Rmet_6206, cnrR, cnrX
Sequence domains: Heavy-metal resistance
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

3 bound ligands:
Ligand ZN 3 x ZN
Ligand CL 3 x CL
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 31.896Å b: 81.809Å c: 93.336Å
α: 90° β: 90.47° γ: 90°
R-values:
R R work R free
0.19 0.187 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Signaling diversity and evolution of extracytoplasmic function (ECF) σ factors.
Mascher T. (2013)
6 more