› 2y5d

X-ray diffraction
1.4Å resolution

Crystal structure of C296A mutant of the box pathway encoded ALDH from Burkholderia xenovorans LB400

Released:
DOI: 10.2210/pdb2y5d/pdb
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Source organism: Paraburkholderia xenovorans LB400
Primary publication:
Elucidating the reaction mechanism of the benzoate oxidation pathway encoded aldehyde dehydrogenase from Burkholderia xenovorans LB400.
Bains J, Leon R, Temke KG, Boulanger MJ
Protein Sci 20 1048-59 (2011)
PMID: 21495107

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 534 amino acids
Theoretical weight: 55.81 KDa
Source organism: Paraburkholderia xenovorans LB400
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q13WK4 (Residues: 1-531; Coverage: 100%)
Gene name: Bxe_A1420
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand P6G 2 x P6G
Ligand NAP 2 x NAP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P1
Unit cell:
a: 58Å b: 67.7Å c: 77.54Å
α: 111.09° β: 90.62° γ: 113.36°
R-values:
R R work R free
0.162 0.16 0.193
Expression system: Escherichia coli BL21

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Citations

3 citation in other articles

Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species.
Hettle et al. (2019)
2 more

2 mentions without citation

A perspective on the PDB's impact on the field of glycobiology.
Prestegard JH. (2021)
1 more

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