› 2y6q

X-ray diffraction
2.37Å resolution

Structure of the TetX monooxygenase in complex with the substrate 7- Iodtetracycline

Released:
DOI: 10.2210/pdb2y6q/pdb
PDB entry 2y6q coloured by chain, front view.
PDB entry 2y6q coloured by chain, side view.
PDB entry 2y6q coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron
Primary publication:
Structural basis for a new tetracycline resistance mechanism relying on the TetX monooxygenase.
Volkers G, Palm GJ, Weiss MS, Wright GD, Hinrichs W
FEBS Lett 585 1061-6 (2011)
PMID: 21402075

Function and Biology Details

Reaction catalysed: 
Tetracycline + NADPH + O(2) = 11a-hydroxytetracycline + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin-dependent monooxygenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 398 amino acids
Theoretical weight: 44.7 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93L51 (Residues: 11-388; Coverage: 97%)
Gene name: tetX2
Sequence domains: FAD binding domain
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
2 bound ligands:
Ligand I7T 4 x I7T
Ligand SO4 12 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.3
Spacegroup: P1
Unit cell:
a: 68.7Å b: 79.96Å c: 88Å
α: 111.1° β: 90.41° γ: 92.96°
R-values:
R R work R free
0.219 0.218 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Ribosome-targeting antibiotics and mechanisms of bacterial resistance.
Wilson DN. (2014)
12 more

3 mentions without citation

Plasticity, dynamics, and inhibition of emerging tetracycline resistance enzymes.
Park et al. (2017)
2 more