Function and Biology Details
Reactions catalysed:
Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- B-form DNA mimic Ocr
- S-adenosyl-L-methionine-dependent methyltransferase superfamily
- Protein Ocr
- DNA methylase, N-6 adenine-specific, conserved site
- DNA methylase, adenine-specific
- N6 adenine-specific DNA methyltransferase, N-terminal domain
- Type I restriction enzyme EcoKI-like, methylase subunit, N-terminal domain superfamily
- Type I restriction modification DNA specificity domain
1 more domain
Structure analysis Details
Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-137261 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Type I restriction enzyme EcoKI specificity subunit
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 51.47 KDa
Source organism: Escherichia coli
UniProt:
Sequence domains: Type I restriction modification DNA specificity domain
Length: 464 amino acids
Theoretical weight: 51.47 KDa
Source organism: Escherichia coli
UniProt:
- Canonical:
P05719 (Residues: 1-464; Coverage: 100%)
Sequence domains: Type I restriction modification DNA specificity domain
Type I restriction enzyme EcoKI methylase subunit
Molecule details ›
Protein Ocr
Molecule details ›
Chains: D, E
Length: 116 amino acids
Theoretical weight: 13.69 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
Sequence domains: DNA mimic ocr
Length: 116 amino acids
Theoretical weight: 13.69 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
- Canonical: P03775 (Residues: 1-116; Coverage: 99%)
Sequence domains: DNA mimic ocr
