› 2y7g

X-ray diffraction
1.4Å resolution

Crystal structure of the 3-keto-5-aminohexanoate cleavage enzyme (Kce) from C. Cloacamonas acidaminovorans in complex with the product acetoacetate

Released:
DOI: 10.2210/pdb2y7g/pdb
PDB entry 2y7g coloured by chain, front view.
PDB entry 2y7g coloured by chain, side view.
PDB entry 2y7g coloured by chain, top view.
Source organism: Candidatus Cloacimonas acidaminovorans
Primary publication:
3-Keto-5-aminohexanoate cleavage enzyme: a common fold for an uncommon Claisen-type condensation.
Bellinzoni M, Bastard K, Perret A, Zaparucha A, Perchat N, Vergne C, Wagner T, de Melo-Minardi RC, Artiguenave F, Cohen GN, Weissenbach J, Salanoubat M, Alzari PM
J Biol Chem 286 27399-405 (2011)
PMID: 21632536

Function and Biology Details

Reaction catalysed: 
(5S)-5-amino-3-oxohexanoate + acetyl-CoA = L-3-aminobutanoyl-CoA + acetoacetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109245 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-keto-5-aminohexanoate cleavage enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 31.09 KDa
Source organism: Candidatus Cloacimonas acidaminovorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0VHH0 (Residues: 2-276; Coverage: 100%)
Gene names: CLOAM0912, kce
Sequence domains: beta-keto acid cleavage enzyme
Structure domains: Aldolase class I

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand AAE 2 x AAE
Ligand GOL 1 x GOL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P43212
Unit cell:
a: 102.1Å b: 102.1Å c: 100.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Recent advances in biocatalyst discovery, development and applications.
Yang et al. (2014)
7 other articles

1 mention without citation

3-Keto-5-aminohexanoate cleavage enzyme: a common fold for an uncommon Claisen-type condensation.
Bellinzoni et al. (2011)