› 2y88

X-ray diffraction
1.33Å resolution

CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS PHOSPHORIBOSYL ISOMERASE (VARIANT D11N) WITH BOUND PRFAR

Released:
DOI: 10.2210/pdb2y88/pdb
PDB entry 2y88 coloured by chain, front view.
PDB entry 2y88 coloured by chain, side view.
PDB entry 2y88 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Bisubstrate specificity in histidine/tryptophan biosynthesis isomerase from Mycobacterium tuberculosis by active site metamorphosis.
Due AV, Kuper J, Geerlof A, von Kries JP, Wilmanns M
Proc Natl Acad Sci U S A 108 3554-9 (2011)
PMID: 21321225

Function and Biology Details

Reactions catalysed: 
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosyl isomerase A Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 25.66 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WMM5 (Residues: 1-244; Coverage: 100%)
Gene names: MTCY336.01c, MTV046.01, Rv1603, hisA, priA
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand 2ER 1 x 2ER
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P43212
Unit cell:
a: 63.27Å b: 63.27Å c: 131.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.138 0.184
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Emerging Targets in Photopharmacology.
Lerch et al. (2016)
5 more

7 mentions without citation

The tryptophan biosynthetic pathway is essential for Mycobacterium tuberculosis to cause disease.
Lott JS. (2020)
6 more