› 2y8p

X-ray diffraction
2Å resolution

Crystal Structure of an Outer Membrane-Anchored Endolytic Peptidoglycan Lytic Transglycosylase (MltE) from Escherichia coli

Released:
DOI: 10.2210/pdb2y8p/pdb
PDB entry 2y8p coloured by chain, front view.
PDB entry 2y8p coloured by chain, side view.
PDB entry 2y8p coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
High-resolution crystal structure of MltE, an outer membrane-anchored endolytic peptidoglycan lytic transglycosylase from Escherichia coli.
Artola-Recolons C, Carrasco-López C, Llarrull LI, Kumarasiri M, Lastochkin E, Martínez de Ilarduya I, Meindl K, Usón I, Mobashery S, Hermoso JA
Biochemistry 50 2384-6 (2011)
PMID: 21341761

Function and Biology Details

Reaction catalysed: 
Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143188 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-type membrane-bound lytic murein transglycosylase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 21.41 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0C960 (Residues: 19-203; Coverage: 98%)
Gene names: JW5821, b1193, emtA, mltE, sltZ, ycgP
Sequence domains: Transglycosylase SLT domain
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2221
Unit cell:
a: 123.324Å b: 183.932Å c: 35.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.252
Expression system: Escherichia coli K-12

Quick links

Citations

8 review citations

The physiology of bacterial cell division.
Egan et al. (2013)
7 more

10 mentions without citation

Lytic transglycosylases: concinnity in concision of the bacterial cell wall.
Dik et al. (2017)
9 more