› 2y9h

X-ray diffraction
2.5Å resolution

Structure A of CRISPR endoribonuclease Cse3 bound to 19 nt RNA

Released:
DOI: 10.2210/pdb2y9h/pdb
PDB entry 2y9h coloured by chain, front view.
PDB entry 2y9h coloured by chain, side view.
PDB entry 2y9h coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3.
Sashital DG, Jinek M, Doudna JA
Nat Struct Mol Biol 18 680-7 (2011)
PMID: 21572442

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176181 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endoribonuclease Cse3 Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 215 amino acids
Theoretical weight: 24.05 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53WG9 (Residues: 1-211; Coverage: 100%)
Gene names: TTHB192, cas6, cse3
Sequence domains: CRISPR associated protein
Structure domains:
5'-R(*UP*CP*CP*CP*CP*AP*CP*GP*CP*GP*UP*GP*UP*GP *GP*GP*DGP*AP*UP)-3' Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 19 nucleotides
Theoretical weight: 6.07 KDa
Source organism: Thermus thermophilus HB8
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 85.86Å b: 149.81Å c: 87.26Å
α: 90° β: 94.6° γ: 90°
R-values:
R R work R free
0.232 0.23 0.29
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

38 review citations

RNA-guided genetic silencing systems in bacteria and archaea.
Wiedenheft et al. (2012)
37 more

1 mention without citation

Structural Principles of CRISPR RNA Processing.
Li H. (2015)