› 2yal

X-ray diffraction
2.27Å resolution

SinR, Master Regulator of biofilm formation in Bacillus subtilis

Released:
DOI: 10.2210/pdb2yal/pdb
PDB entry 2yal coloured by chain, front view.
PDB entry 2yal coloured by chain, side view.
PDB entry 2yal coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure and organisation of SinR, the master regulator of biofilm formation in Bacillus subtilis.
Colledge VL, Fogg MJ, Levdikov VM, Leech A, Dodson EJ, Wilkinson AJ
J Mol Biol 411 597-613 (2011)
PMID: 21708175

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator SinR Chains: A, B
Molecule details ›
Chains: A, B
Length: 41 amino acids
Theoretical weight: 4.94 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06533 (Residues: 75-111; Coverage: 33%)
Gene names: BSU24610, flaD, sin, sinR
Sequence domains: Anti-repressor SinI

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P6122
Unit cell:
a: 36.117Å b: 36.117Å c: 250.342Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.289 0.283 0.435
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 citation in other articles

Characterisation of Clostridium difficile biofilm formation, a role for Spo0A.
Dawson et al. (2012)
24 more

5 mentions without citation

Molecular basis of the activity of SinR protein, the master regulator of biofilm formation in Bacillus subtilis.
Newman et al. (2013)
4 more