› 2ycj

X-ray diffraction
1.96Å resolution

methyltransferase bound with methyltetrahydrofolate

Released:
DOI: 10.2210/pdb2ycj/pdb
PDB entry 2ycj coloured by chain, front view.
PDB entry 2ycj coloured by chain, side view.
PDB entry 2ycj coloured by chain, top view.
Source organism: Carboxydothermus hydrogenoformans Z-2901
Primary publication:
Structural basis for electron and methyl-group transfer in a methyltransferase system operating in the reductive acetyl-CoA pathway.
Goetzl S, Jeoung JH, Hennig SE, Dobbek H
J Mol Biol 411 96-109 (2011)
PMID: 21640123

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174498 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pterin-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 30.17 KDa
Source organism: Carboxydothermus hydrogenoformans Z-2901
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3ACR9 (Residues: 1-263; Coverage: 100%)
Gene names: CHY_1227, acsE
Sequence domains: Pterin binding enzyme
Structure domains: Dihydropteroate synthase-like

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 3 x GOL
Ligand C2F 1 x C2F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P43212
Unit cell:
a: 64.702Å b: 64.702Å c: 177.302Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 citation in other articles

The genetic basis for bacterial mercury methylation.
Parks et al. (2013)
14 more

1 mention without citation

Insights into pyrrolysine function from structures of a trimethylamine methyltransferase and its corrinoid protein complex.
Li et al. (2023)