› 2yck

X-ray diffraction
2.15Å resolution

methyltransferase bound with tetrahydrofolate

Released:
DOI: 10.2210/pdb2yck/pdb
PDB entry 2yck coloured by chain, front view.
PDB entry 2yck coloured by chain, side view.
PDB entry 2yck coloured by chain, top view.
Source organism: Carboxydothermus hydrogenoformans Z-2901
Primary publication:
Structural basis for electron and methyl-group transfer in a methyltransferase system operating in the reductive acetyl-CoA pathway.
Goetzl S, Jeoung JH, Hennig SE, Dobbek H
J Mol Biol 411 96-109 (2011)
PMID: 21640123

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174498 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pterin-binding domain-containing protein Chain: X
Molecule details ›
Chain: X
Length: 272 amino acids
Theoretical weight: 30.22 KDa
Source organism: Carboxydothermus hydrogenoformans Z-2901
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3ACR9 (Residues: 1-263; Coverage: 100%)
Gene names: CHY_1227, acsE
Sequence domains: Pterin binding enzyme
Structure domains: Dihydropteroate synthase-like

Ligands and Environments

3 bound ligands:
Ligand SO4 7 x SO4
Ligand GOL 1 x GOL
Ligand THG 1 x THG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P43212
Unit cell:
a: 63.955Å b: 63.955Å c: 175.095Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 citation in other articles

The genetic basis for bacterial mercury methylation.
Parks et al. (2013)
15 more